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Poyser said he hoped the same approach could be used if a complete profile of the Zodiac is obtained. It's a 50-years-old case." The use of public genealogy sites to track criminal DNA is relatively new.

"There are probably 30 different circumstantial things that point to him. He was extremely intelligent but a deviant dude." Allen largely was cleared as a suspect in 2002, when investigators obtained a partial DNA sample from the envelopes that didn't match his.

The Zodiac Killer roamed Northern California from December 1968 through October 1969, but was never caught despite at least one close run-in with police.

He is known to have attacked seven victims — in Benicia, Vallejo, Lake Berryessa and San Francisco — killing five and leaving two survivors.

"They were confident they would be able to get something off it," Poyser said.

He said the envelopes had been sent to the lab a few months ago, before Joseph De Angelo, who is suspected of being the East Area Rapist, was caught after investigators used a DNA profile run though an open-source database.